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Planting and processing of earthworms (1)
According to the experience of the old tenant farmers, “If you want to burst your hair, you must prepare a winter wax, you can only sweep your tail in the first month.†Therefore, it is time to prepare for planting. Amaranthus is a Polyporaceae plant, used for sclerotia for medicinal purposes, is a commonly used Chinese herbal medicine, and is mainly produced in Anhui, Fujian, Hubei, Hunan, and other provinces. It enjoys a warm, dry, ventilated, sunny, abundant rainfall environment. The required slope is about 10-30 degrees, and the soil is sandy loam, fertile and moist environment. Wild clams usually grow above 500 meters above sea level and grow on the roots of decayed pine trees or pine trees, about 20 centimeters underground. The growth and development of earthworm can be divided into two stages, the mycelium growth stage and the sclerotium formation stage. The mycelial growth stage is mainly the hyphae absorbing moisture and nutrients from the pine wood, and multiplying the vegetative mycelium. When the mycelium grows more and more, it becomes a sclerotia, and when the sclerotia stage is formed, the cultivation is called the scab phase. Artificially cultivated alfalfa is currently commonly used as a cultivation method for the cellar cultivation method. First, the pure alfalfa strain should be cultivated, and then the digging can be performed again. Cultivation of alfalfa pure cultures (1) Preparation of culture medium for female parent: Potato-agar medium is mostly used. Its formula is 250 grams of potato, 50 grams of sucrose, 20 grams of agar, 3 grams of urea, and 1000 milliliters of water; Prepare the solution by conventional methods, adjust the pH to 6-7, aliquot the tube, bund, sterilize it, and make it a slant medium. Separation and Inoculation of Pure Bacteria: Select fresh, thin skin, white flesh, tight and fragrant mature sclerotium nucleus as the species, first wash with water, and then move into the inoculation box; 75% of the alcohol is washed, Rinse it several times with distilled water or cold water, dry it with filter paper, cut with a knife, pick up a small piece of central white meat, place it on the slope of the medium, and place it in a 25-30 degree incubator or incubator In cultivation for 5-7 days until the white mycelium is covered with a slant, it is purebred. (2) After the successful cultivation of the purebred mother of the original species can not be directly used for production, but also to expand the breeding and breeding of the original species. The original culture medium formula: 55% of pine wood blocks, 20% of pine wood chips, 20% of rice bran or wheat bran, 4% of sucrose, 1% of gypsum powder; add appropriate amount of water, and adjust PH value to 5-6. Preparation method: first wood chips, rice bran, gypsum powder and mix; the other add sucrose water-soluble, put pine wood block boil for 30 minutes, until the pine wood block fully absorb the sugar liquid, remove; then the shavings, rice sugar, gypsum powder Pour into the liquid, mix well; then add the wood, mix well, and use the fingers to squeeze the water for the degree. It is then dispensed into 500 ml jars, sterilized and allowed to inoculate after cooling. Inoculation method: Under aseptic conditions, the mother strains of soybean size were selected from the parent species and placed in the center of the original species of the wide-mouth bottle, which was the original species. (3) Cultivation of cultivated species Culturing culture medium consists of wood 66%, pine wood chips 10%, wheat bran or fine mash 21%, glucose 2%, gypsum powder 1%, urea 0.4%, calcium superphosphate 1% , add water, adjust the PH value of 5-6. The preparation method is the same as above. The mycelium in the original species was received in a wide-mouth bottle with culture material, and the mycelium covered the whole bottle in about 30 days. One bottle of pure seed can be sorted by 5-8 bottles, one bottle of original can be bred by 60 bottles, and one bottle of cultivar can be connected by 2-3 cellar. Selection of cellar and pine wood selection of deep and loose soil, well-drained sandy loam, sunny slopes and uncultivated raw land, woodland, winter will cellar dug good, cell length 80 cm, width 45 cm, depth 30 In centimeters, the distance between the pit and the pit is 30 cm, and a drainage ditch is opened. Using pine wood as a cultivation material, in the lunar calendar in December, the pines were cut down, the tree shrews were cut, the layered skins were cut off, the xylem was exposed, and the ribs were peeled and sawn into a 60-80 cm long section of wood. After stacking and drying, they were dried. After 40 days, there is no turpentine secretion, and the wood heart can be inoculated after drying.