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Preservation of edible mushroom strains
The decline of edible fungi strains is a common phenomenon. Adopting suitable preservation methods and minimizing the passage times of strains is one of the effective measures to prevent the decline of strains. The preservation principle of strains is to reduce their metabolic effects, and there are many methods for preservation. Two types of strains are mainly introduced for reference by the whole city. The first is the oblique cryopreservation method: This is the simplest method of preservation, that is, the growth of pollution-free bevel tube into the refrigerator, stored at a low temperature of 4-6 °C, after every 2-3 months transfer In fresh slant culture medium, continue to preserve after cultivation (to supplement nutrients and moisture of hyphae to prevent hyphae aging). The second is liquid paraffin preservation method: this method is to store the paraffin in the sterile chamber in the beveled tube, injection wax to 1 cm above the slope is appropriate. Its purpose is to isolate bacteria from the air and reduce its metabolic activity. Then stuffed with a cork or rubber stopper, sealed with wax, upright placed in a dry place indoors, can be stored for more than one year. When using, only a small amount of mycelium is picked from the slant with an inoculation needle and transplanted on a fresh slant medium. Since the hyphae are stained with paraffin, it must be transferred to a test tube to grow well.