Recently, Plant Cell and Environment published a research paper entitled "The B-Box zinc finger protein MdBBX20 integrates anthocyanin accumulation in response to ultraviolet radiation and low temperature" by the State Key Laboratory of Crop Biology, Shandong Agricultural University. The study found that the BBX20 gene in apples promotes the synthesis of anthocyanins in apple peels by responding to UV-B and low temperatures. It is worth mentioning that Plant and Cell Physiology also published a research paper entitled “MdCOL4 Interaction Mediates Crosstalk Between UV-B and High Temperature to Control Fruit Coloration in Apple†in the previous issue. Both articles revealed BBX transcription. Factor-mediated UV-B and temperature regulation of the synthesis of anthocyanins in apple peels. In this study, the differential expression of the BBX transcription factor IV subfamily members under UV-B conditions was screened to obtain the differentially expressed gene MdBBX20. In addition to responding to UV-B, we found a low-temperature response element LTR on the promoter of MdBBX20 in order to explore the role of MdBBX20 in the low-temperature signaling pathway. MdBBX20 was overexpressed in the callus of apple grove, and cultured under different UV-B and temperature conditions. It was found that the callus of Wanglin overexpressing MdBBX20 accumulated the most anthocyanins under UV-B/low temperature conditions. Interestingly, Even without UV-B, Wang Lin callus overexpressing MdBBX20 can accumulate anthocyanins only at low temperatures. This indicates that MdBBX20 can promote the synthesis of anthocyanins in response to UV-B and low temperature. At the same time, it was found by the yeast mono- and EMSA assay that MdbHLH3 recognizes and binds to the LTR cis-acting element on the MdBBX20 promoter. It is further explained that MdBBX20 can promote the synthesis of anthocyanins in response to low temperature with the participation of MdbHLH3. At the same time, yeast single-hybrid, EMSA and CHIP experiments confirmed that MdBBX20 can specifically recognize and bind to the G-box of MdMYB1 and downstream structural genes MdDFR and MdANS promoter, and promote its expression. Previous studies have shown that HY5 plays an important role in the coordinated regulation of UV-B and temperature signaling pathways. In this study, yeast two-hybrid assay, bimolecular fluorescence complementation assay and Pull down assay were used to verify that MdBBX20 interacts with MdHY5 to form a protein complex. By decomposing the domain, the second B-box domain of MdBBX20 and the b-ZIP domain of MdHY5 are required for the interaction between the two. The results of LUC fluorescence reporter experiments indicated that MdBBX20-MdHY5 MdBBX20 can promote the binding of MdHY5 to the MdMYB1 promoter. This shows that MdBBX20 can coordinate the two signal pathways of UV-B and low temperature through interaction with MdHY5. Professor Chen Xuesen and Dr. Wang Nan from Shandong Agricultural University are the authors of this article. The first author is Dr. Fang Hongcheng from Shandong Agricultural University. Professor Wu Shujing from Shandong Agricultural University and Li Jianmin, director of the Jingning Fruit Tree Institute of Gansu Province, provided relevant guidance and assistance to the research. The research was funded by national key R&D projects and key projects of the National Natural Science Foundation.
The Medical Bed Head Unit is made of aluminum alloy sections, of which the surface is treated with acrylic painting or electrostatic spray. It is equipped with the dismountable panel, three built-in cabling channels for gas, strong electricity and weak electricity, and external leakage protectors, bedside lamps, large toggle switches, five-hole multi-function socket, gas terminal and Medical wards calling system. The hospital can choose the appropriate color in line with the environmental requirements.
Bed Head Unit For Ward Bed Head Unit For Ward ,Bed Head Panel ,Hospital Bed Head Panel,Mural Bed Head Unit Hunan Eter Electronic Medical Project Stock Co., Ltd. , http://www.centralgas.be