Release date: 2007-11-05 "Brain Rainbow" technology helps to build a detailed diagram of the brain neural network. With the introduction of genes for bacteria, corals and jellyfish, scientists have colored the original red brain of the experimental mice to clarify the main lines and branches of the nerve brain cells. The new technology, called Brain Rainbow, was published in the November 1 issue of Nature. Its inventor, Harvard University experts believe that this will enable scientists to build detailed charts of brain neural networks that can help in-depth study of how the brain works.
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Prior to this, the technique of displaying neuronal cells could only use up to two colors, which is the Golgi staining method. The Golgi staining method still in use today was founded and developed by Italian physicians in 1876, which applied all neuronal cells to pigments each time, but the result was that only a small fraction of them were successfully stained.
In contrast, the “brain rainbow†technology allows researchers to successfully apply more than 90 distinct colors to hundreds of neuronal cells at a time. Using genetic recombination technology, the researchers first packaged the pigment gene and DNA and inserted it into the cell genome that can be bred into mice. As the cells divide and the mouse embryos form, these pigment genes also divide and grow. To activate these pigment genes, the researchers then implanted a recombinant gene from bacteria into mouse cells. By using pigment genes in different parts of the mouse or at different stages of development, scientists can apply different colors to different types of cells.
Research team member Jeff Lichman explained that the "brain rainbow" technology is just like a color TV display that uses red, green and blue to create a variety of colors, three or more fluorescent colors in neuronal cells. Proteins can produce a variety of colors through chemical combination.†The difference is that “brain rainbow†technology uses three kinds of genetic pigments: “cyan, red and yellowâ€. The red gene pigment is derived from coral, while the green pigment and blue pigment are processed from the green fluorescent pigment in the jellyfish. The sliced ​​picture after dyeing looks like an abstract picture, which is both beautiful and scientific. The team revealed that they have used the technique to observe the nervous system of mice and observed some interesting and undiscovered patterns of neuronal cell distribution.
For the higher base method, the "brain rainbow" technology also has some shortcomings. The brains of mice that have been given "brain rainbow" still look the same as normal, and those rich colors must appear under a fluorescent microscope. This microscope is very expensive and worth hundreds of thousands of dollars, while the Golgi method uses stained slices. Another microscope can be observed; another limitation is that the technology can only be applied to genetically modified or genetically modified animals. Currently, scientists choose only mice, and the Golgi method is applicable to any animal, including humans.
Despite these shortcomings, the “brain rainbow†technology shows neuron cytologists a more complete picture of the brain.
——China Medicine 123 Network