Abstract: Clenbuterol is a class of animal drugs. Several drugs are called clenbuterol, such as ractopamine and clenbuterol. Adding lean meat to the feed can increase the lean meat content of the animal, reduce the use of the feed, bring the meat to the market, and reduce the cost.

The harm of clenbuterol: Clenbuterol, refers to a class of animal drugs, including clenbuterol hydrochloride, ractopamine, salbutamol and terbutaline sulfate, etc., belonging to the adrenal nerve stimulant. Adding "Clenbuterol" to the feed does increase the lean meat content of the animal. However, relevant scientific research at home and abroad has shown that eating meat containing "clenbuterol" can cause harm to the human body. Common symptoms such as nausea, dizziness, weakness of the limbs, and hand tremors are particularly harmful to patients with heart disease and hypertension. Big. Long-term consumption may lead to chromosomal aberrations, which may induce malignant tumors. As for how much intake is high, how to cause malignant tumors, the research on related cases is still inconclusive. However, in recent years, cases of poisoning and even death caused by “clenbuterol” have occurred from time to time.

Detection method: Sensory identification method--Chenlenol hydrochloride is more serious. After slaughter, the meat color is particularly bright red, the hip muscles are full and the fat is very thin, and the healthy meat is light red and the meat is elastic.

Chemical analysis - normal fresh meat test paper test its pH is mostly neutral or weakly alkaline, while pork containing clenbuterol is acidic, the pH value is significantly less than the normal range, so the pH method can also be measured. (Inconsistent impurity interference)

Enzyme-linked immunosorbent assay - suitable for rapid analysis of large-scale samples of live animals, but can not achieve on-site testing, no introduction.

Chromatographic techniques - chromatographic techniques including high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), gas chromatography-Fourier infrared spectroscopy (GC-FTIR) and thin layer chromatography (TLC) )Wait. Currently, the most widely used are high performance liquid chromatography and gas chromatography-mass spectrometry.

In order to more accurately detect clenbuterol, here we mainly introduce gas chromatography-mass spectrometry.

The sample (animal urine) was extracted in a buffer solution of pH = 5.2. The extracted sample was purified by solid phase extraction using C18 and SCX cartridges. The separated drug residues were derivatized with bistrimethylsilyltrifluoroacetamide (BSTFA) and determined by Linghua gas chromatograph with mass selective detector. .

Advantages: high detection accuracy, high accuracy, high efficiency, good reproducibility, high sensitivity, low false positive rate