First, waste collection and Agaricus bisporus cultivation material formula Generally in the middle of April, the mushroom production is completed, its cultivation waste is taken out of the bag and dried, and the combination of drying and tapping is used to crush and concentrate to prevent rain. Bisporus sulphate cultivation material formula: calculate the dry weight of mushroom waste, add 3% superphosphate, 2.5% quicklime powder, 1.5% gypsum powder, 4% corn flour, 15% fresh cottonseed husk, then add 8% to 10% Euphorbia or rapeseed husk to improve the ventilation of the material. Second, the heap fermentation The bagged mushroom bisporus culture material is composted at the time of composting, and the moisture content of the material is adjusted to 65% to 70%, and the composting is carried out for 15 to 20 days, during which the temperature in the waiting pile naturally rises to 60~ When the temperature is 70°C, it is necessary to pay attention to the up and down of the material, change the position inside and outside to ensure that the fermentation is even and turn it 2 or 3 times. Third, bagging sterilization After the completion of the composting process, the diluted culture materials will be exhausted and the water content of the adjustment material will be about 60%. The use of old bags longer than the needle mushroom bagging, the general bag size is 19 cm 33 cm low pressure polyethylene bags. When loading, press the fist with your hand, or use the machine to bag, reach the appropriate degree of tightness, and then conduct normal pressure steam sterilization for 8 to 10 hours. IV. Inoculation and bacterial administration Sterilization is complete. When the material temperature in the bag is lower than 28°C, each bottle can be inoculated with 20-22 bags of culture material. Inoculation boxes are generally used in summer and open vaccination can be performed in early spring and late autumn. Before the inoculation, the site was sprinkled with a mixture of lime powder and gemma net, in a ratio of 100:1, and 0.5% gemmyc was sprayed into the space or the air was purified. After inoculation, strains were cultured and cultured in sterile bags. In summer, the bacteria bags are discharged in a single layer and the roof of the greenhouse is covered with a shade net to cool the temperature; in early spring and late autumn, 3 to 4 layers can be stacked, and the double-membrane greenhouse plastic film + straw curtain + plastic film can be used for warming and heat preservation, timely ventilation, and protection from light. Under the promotion of hyphae germination is fast, after about 50 days, mycelium can be filled with bags. Fifth, take off bag cover soil According to the weather and the market price of fresh mushrooms to grasp the bag-sanding and covering time, tear the film of the bag and take it off, place it on the ground or on the bed frame, cover the mixed soil of soy soil, adjust the pH value of soil particles to 7.5 ,Using 5% formaldehyde solution evenly sprayed soil particles, and cover the film for 24 hours to disinfect the membrane to disperse the odor. Transfer the treated soil particles to the state where the water is squeezed into the dough and the ground is scattered. When filling the soil, fill the soil. With full crevices, the thickness of the mixture of material surface thickness is preferably 3 to 4 cm. Sixth, water transfer mushroom When the mycelium is stretched to 2/3 of the covering layer, the temperature in the shed is generally controlled at 25°C in the late summer and early autumn in the early summer; in early winter, the temperature is above 10°C, and the mushroom water must be sprayed in a timely and accurate manner to promote the kinking of the mycelium and the water spray volume is Usually 2 to 3 times, with enough moisture to absorb the soil. When spraying mushroom water, ventilation must be increased more than usual. When the size of the bean sprouts appeared in the earth seam, mushroom water was sprayed in time to promote fruit body formation. During mushroom harvesting, indoor relative humidity is kept between 90% and 95%. The amount of water spray should be controlled flexibly according to the amount of mushrooms and weather conditions. Light spray, ground spray, and intermittent water spray are the main factors. Mushrooms are sprayed more and more. Multi-spray, less rainy days or not spray, avoid playing closed water, bogey in the indoor high temperature and before the mushroom spray, each wave mushroom appropriate increase in early ventilation, but need to maintain the mushroom shed relative humidity of about 90%, the latter mushroom Reduce the amount of ventilation properly, the entire cultivation and management process, and correctly handle the relationship between water spray, ventilation, and moisturizing, not only to increase the number of mushrooms, produce good mushrooms, but also to protect the hyphae, promote the vigorous growth of hyphae, vigorously in the middle period, and late Not premature aging. 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