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1. Do a good job in environmental sanitation and keep the inoculation room and inoculation box clean, dry and sterilized.
2. The surrounding environment should minimize pollution and clean up routinely.
3. The strain production area shall establish personnel and goods import and export health management systems and use clean transportation tools.
4. Inoculation tools and operators perform the necessary disinfection.
(B) Preparation of good media
1. The formula of the culture medium is reasonable, the culture material is fresh, no mildew, and no sharp hard materials.
2. Adding 0.1% to 0.2% of carbendazim when mixing spices, can inhibit the growth of bacteria.
3. Mixing materials should be evenly distributed, and the culture materials should be broken up to form a mass. The water content should be appropriate. The general moisture content is 60%-65%.
4. The bottling (bags) should be moderately tight, and the strains should be tightly closed after plugging the tampon.
5. Timely sterilization, generally not overnight.
6. Bacteria species bottle mouthwash culture materials to prevent contamination.
(three) control the tampon dampness
1. Culture materials should not be too large water content, loading should not be too full.
2. When sterilizing, the cold air is drained and the speed cannot be too fast so as to avoid wet tampon.
3. When sterilized, the tampon is wrapped with kraft paper or plastic paper.
4. Damp tampons were found to be replaced in time when inoculated.
(D) strict sterilization
1. Maintain the sterilization temperature and time, sterilize at normal pressure, maintain the temperature at l00°C for 8-10 hours, and maintain the pressure on the autoclave for 1.5-2 hours.
2. Take care when lifting the pot, so as not to break the container.
3. The pots (bags) cannot be squeezed too tightly when filled with pots, and appropriate gaps are left to facilitate steam penetration and sterilization.
4. During the sterilization process, cold water should not be added. Warm water or hot water must be added to avoid a sudden drop in the pot temperature.
5. Pay attention to the deflation rate so as not to burst the container.
6. It is better to keep the temperature for 12 hours after the sterilization is finished. Do not immediately open the pan or use a batch sterilization method.
(five) Carefully select the source of the species
1. Purchasing strains should be purchased in units or individuals with sound production management, good detection technology, and good reputation.
2. Try to understand the biological characteristics of the strain you choose to avoid blind production.
3. Select high-quality strains: white, thick, vigorous growth, no impurities, and the best bacteria age.
4. Check carefully before inoculation, conduct surface disinfection, and dig up the old bacteria.
(6) Strict asepsis during inoculation
1. The space environment for inoculation must be strictly purified and disinfected.
2. Inoculation tools, operator's hands, clothes must also be disinfected.
3. Do not leave the sterile area of ​​the flame of the alcohol lamp when inoculating.
4. The inoculation moves quickly and quickly, reducing the chance of contamination with germs.
5. Appropriate increase in the amount of inoculation, favorable mycelium germination.
6. Try to avoid inoculation at high temperatures, choose to inoculate at night or early morning
(VII) Strengthening the cultivation and management of strains
1. Do a good job in the cleanliness of the training room and rigorously sterilize it before use.
2. Clean up contaminated strains in time and isolate them to prevent the spread of germs.
3. Avoid high temperature and high humidity during the cultivation, the temperature of the culture room is generally 22-25 degrees, and the relative humidity of the air is generally 60%-70%.
4. Pay attention to the ventilation in the culture room and keep the air fresh.
5. The growth of Ganoderma lucidum mycelium does not require light, so the light in the culture room should not be too strong. It is best to keep the yin and yin yang at seven points for the growth of mycelium.
Ganoderma lucidum strain pest control measures
(I) Do a good job in environmental purification