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Genetically modified potato anti-roll leaf virus
The potato transformed with the coat protein gene of the Chinese potato leafroll virus (Chinese strain) that was cloned for the first time was introduced at Inner Mongolia University. At the appraisal meeting organized by the Ministry of Science and Technology of Inner Mongolia on December 5, experts agreed that the results accomplished by Professor Zhang Heling from Inner Mongolia University were at the international advanced level. Potato leafroll virus is the main virus that causes potato degeneration. Severe infections can lead to a reduction of 80% of production. Each year, the world potato production is reduced by about 2 million tons. Under the auspices of Prof. Zhang Heling from Inner Mongolia University, in 1993, the National Natural Science Foundation Project “Cloning of coat protein genes of potato leaf virus†was completed. Afterwards, they used the coat protein gene of their composita cloned potato leafroll virus (Chinese strain) through Agrobacterium-mediated transformation. After several years of breeding, they obtained five cultivars with stable cultivars. A total of 20 strains. In the study, a set of molecular biology and genetic engineering experimental techniques was established in the laboratory by scientists and technicians from Inner Mongolia University, where Professor Zhang Heling was the principal. Create varieties of resources that have value for promotion and application in production. Compared with the control, potato leafroll virus can be reduced by more than 50%. Prof. Zhang Heling and others conducted community experiments this year. The average yield of genetically modified potato of various varieties increased by more than 30%, and the maximum yield per mu was 4200 kg. The quality of genetically modified potatoes is comparable to that of potatoes without genetically modified plants. Through research, Prof. Zhang Heling creatively established a highly efficient Agrobacterium-mediated transformation of potato and induced regeneration of plantlets at home and abroad. For the first time, he established the use of leaf-rolling virus replicase gene complementary DNA nucleic acid probes for detection. New Technology for Leafroll Viruses in Coat Protein Gene Plants At the same time, the resistance mechanism of transgenic potato was further discussed. It was first proposed in China for post-transcriptional gene silencing. (Gene silencing refers to a mechanism by which a transferred gene can activate plant cells, which can identify Genes can also identify viral genes and can be degraded at the same time for antiviral purposes).