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Bovine serum albumin (BSA) is a globulin in bovine serum containing 607 amino acid residues with a molecular weight of 66.446 KDa and an isoelectric point of 4.7. Bovine serum albumin has a wide range of applications in biochemical experiments. According to the grade from low to high, it is divided into: bovine serum albumin, bovine serum albumin fifth component, bovine serum albumin (without essential fatty acids), bovine serum albumin (no protease), bovine serum albumin (cell culture grade). ), gold standard bovine serum albumin, cross-linked bovine serum albumin. The customer needs to select the appropriate level of bovine serum albumin according to the actual situation. The following is a brief description of each grade of BSA. |
First, the lowest level BSA |
The main ingredient is BSA products can be bovine serum albumin, generally used in industrial production. |
Second, the fifth component of bovine serum albumin, English abbreviated BSAV |
This is a biotech-grade product with the widest range of applications. Some manufacturers have low levels of BSAV fatty acids and endotoxins and can be used directly in cell culture for high purity and can be used in any relevant experiment. Such as ELISA, WB experiment. |
Third, bovine serum albumin (without essential fatty acids) |
Low fatty acid content, fatty acid content ≤0.002% (W / W), or fatty acid concentration <5umol / l in 1% BSA solution, can be used to determine the concentration of free fatty acids released from cultured tissue cells. |
Fourth, bovine serum albumin (no protease) |
Or called Protease bovine serum albumin, the product does not contain DNAase, protease and IgG, can be used for radioimmunoassay, enzyme-linked immunoassay and as a diluent or blocking agent. |
5. Bovine serum albumin (cell culture grade) |
The purity is 98%, but the fatty acid content and endotoxin content are extremely low, and it is used in cell culture cell culture related experiments. |
Gold standard grade bovine serum albumin |
High purity, purity of 99% or more, used to make gold-labeled antibodies. |
Cross-linked bovine serum albumin |
Freeze-dried powder containing monomer (66000), dimer (132000), trimer (198000), tetramer (264000), and the like. It is used to determine the molecular weight of positional proteins and can be used in biochemical teaching experiments to explore electrophoresis conditions and to determine electrophoresis results. |
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