(1) Operate in strict accordance with the requirements of the fluorescence microscope factory instructions, do not arbitrarily change the program. (2) Inspection should be carried out in a dark room. After entering the dark room, connect the power supply and ignite the ultra-high pressure mercury lamp for 5 to 15 minutes. After the light source is stable, the eyes are completely adapted to the dark room, and then the specimen is observed. (3) To prevent damage to the eyes caused by ultraviolet rays, wear protective glasses when adjusting the light source. (4) The inspection time is preferably 1~2h each time, more than 90min, the luminous intensity of the ultra-high pressure mercury lamp is gradually decreased, and the fluorescence is weakened; after the specimen is exposed to ultraviolet light for 3~5min, the fluorescence is also obviously weakened; therefore, the maximum is not more than 2~ 3h. (5) Fluorescence microscope light source has a limited life, specimens should be intensively checked to save time and protect the light source. When it is hot, the fan should be cooled and cooled down. The new lamp should be recorded from the beginning. When the lamp is to be used again, it must be cooled before the lamp is fully cooled. Avoid igniting the light source several times a day. (6) The specimen is observed immediately after staining, and the fluorescence will gradually weaken due to the long time. If the specimen is stored in a polyethylene plastic bag at 4 ° C, the fluorescence decay time can be delayed and the sealing agent can be prevented from evaporating. (7) Judging criteria for fluorescence brightness: generally divided into four levels, namely "one" - no or visible weak fluorescence. "+" - only visible fluorescence is visible. "++" - visible bright fluorescence. "+++" - visible dazzling fluorescence. Kn95 5 Layers 3D Mask,Kn95 Protective Respirator,Filter Cotton Kn95 Face Mask,Kn95 5 Layers Fold Flat Respirator Jiangmen anjian biotechnology co. LTD , https://www.jmanjianmask.com